NBT/BCIP Substrate Solution(Single Solution)

更新:2021-11-12 16:52:43

中文名:NBT/BCIP Substrate Solution(Single Solution)说明书下载暂无
英文名:NBT/BCIP 底物溶液(单溶液)
描述:Thermo Scientific Pierce NBT 和 BCIP 底物是用于发色印迹和 IHC 染色(使用碱性磷酸酶探针)的硝基四氮唑蓝和 5-溴-4-氯-3'-吲哚基磷酸盐粉末和即用型溶液。
订购信息
    货号规格价格品牌
    E1162-100ml100ml330GBCBIO
    E1162-500ml500ml1380GBCBIO
    E1162-100ml*10100ml*102850GBCBIO
产品介绍

    Application

    A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
    BCIP®/NBT Liquid Substrate System has been used:
    • for staining alkaline phosphatase(ALP) in bone marrow stromal cells
    • as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
    • as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays

    NBT(氯化硝基四氮唑蓝)和 BCIP(5-溴-4-氯-3'-吲哚基磷酸甲苯胺盐)在与碱性磷酸酶(一种常用的抗体探针酶偶联物)反应时产生一种不溶性深黑紫色沉淀物。 NBT 和 BCIP 粉末以两种便利、即用型溶液的形式单独提供(含和不含内源性磷酸酶活性的左旋咪唑抑制剂)。

    特点:
    AP 底物—用于检测固体培养基上的碱性磷酸酶 (alk-phos) 活性,包括硝酸纤维素和 PVDF 膜及固定的组织样品
    • 发色—无需使用特殊设备即可进行可视化;产生易于拍照的紫黑色沉淀物

    NBT/BCIP 单溶液的产品优势:
    • 适用于免疫印迹应用
    • 即用型单一组分
    • 在低背景下具有敏感性并能最小化测定间差异
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